NeuroMab™ Anti-Nogo Antibody(NRP-0422-P1647)

Product Overview

Description

The antibody specifically bound to human Nogo-66 receptor (NgR), also specifically bound to a polypeptide consisting of amino acids of the rat NgR. The antibody have the ability to inhibit the binding of the natural ligand of the Nogo-66 receptor and neutralize the Nogo-66 receptor, are useful for detecting NgR and for inhibiting NgR activity, for example in a human suffering from a disorder in which NgR or Nogo-66 activity is detrimental.

Species Reactivity

Human; Rat

Clonality

Monoclonal

Host Species

Mouse

Applications

ELISA; WB; Inhib; Block; In Vitro; Neut

Relevant Diseases

Alzheimer's Disease; Parkinson's Disease; Multiple Sclerosis; Amyotrophic Lateral Sclerosis

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

Nogo

Official Name

RTN4R

Full Name

Reticulon 4 receptor

Alternative Names

RTN4R; NGR; NOGOR; Reticulon 4 receptor

Gene ID

65078(Human); 65079(Mouse)

Uniprot ID

Q9BZR6(Human); Q99PI8(Mouse)

Product Pictures

ELISA

Figure 1: Antibodies mAb50 and mAb51 binding to rat NgR.

ELISA

Figure 10: Antibodies mAb50 and mAb51 binding to human NgR.

ELISA

Figure 2: AP-Nogo66 competes for binding with mAb50 and mAb51 of NgR-Fc.

ELISA

Figure 3: Nogo66 competes with mAB50 and mAB51 for binding to human and rat NgR expressed on HEK293f cells.

ELISA

Figure 4: Binding of mAB50 and mAB51 to NTera 2 cells.

FuncS

Figure 5: Quantification of neurite outgrowth of NTera 2 cell aggregates.

FuncS

Figure 6: Deletion mutants of hNgR.

FuncS

Figure 7: Competition of MAG-Fc binding to NgR-Fc.

FuncS

Figure 8: Rat dorsal root ganglion neurons under permissive and inhibitory conditions and neutralization of Nogo66-induced inhibition of neurite outgrowth by mAb50.