Organotypic Brain Slices

Organotypic brain slices are powerful and convenient in vitro models in neuroscience research. These slice cultures allow the organizational structure, sophisticated cell populations, and synaptic organization present in vivo to be preserved. Additionally, organotypic brain slices can be maintained for extended periods, allowing for long-term studies of development, plasticity, and disease progression. This model also enables researchers to easily manipulate and control the extracellular environment, making it a valuable tool for studying the effects of drugs, toxins, and other compounds on neural function.

Creative Biolabs is dedicated to offering time and cost-effective organotypic brain slices from rodents with advanced membrane techniques. We take great care in preparing and maintaining organotypic brain slices to ensure optimal viability and functionality for your research needs. Don't hesitate to reach out to us for more information on our organotypic brain slice offerings and how they can benefit your research endeavors.

Applications

Recombinant adeno-associated viruses (rAAV) transduction. Transducing organotypic brain slices with AAV that express α-synuclein or variants of tau can generate reliable neurodegenerative disease models. Transduction with AAV containing broad-spectrum or specific promoters and fluorescent proteins can visualize any protein of interest.
Immunofluorescent labeling for detecting cell types and cell death and viability.
Enzyme-linked immunosorbent assay (ELISA).
Western blot.
Quantitative real-time polymerase chain reaction (qRT-PCR).
Electrophysiological recordings and stimulations.

Research Areas

Neurodegenerative diseases
With viral vector delivery and advanced imaging technology, we can easily obtain neurodegenerative protein-related organotypic brain slice models to study the spreading of these pathogenic proteins.
Angiogenesis of brain vessels
Organotypic brain slices are more representative of in vivo conditions compared to cellular models. This model also contains a complete complement of neuro-vascular unit cells, which is absent in blood flow but still able to respond to stimulation.
Brain tumor cell invasion assay
Emerging evidence highlights the significant impact of the tumor microenvironment and interactions between tumor cells, the extracellular matrix (ECM), and multiple cell types on tumor cell invasion. Organotypic brain slices closely meet this requirement.
Brain aging research
Genome-wide gene expression analyses suggest that organotypic brain slices can mimic the aging process and provide valuable insights into age-related changes in gene expression, which can be drastically accelerated with prions.
Drug screening
Maintained under different culture protocols, organotypic brain slices will respond in many aspects, such as neurogenesis, neuronal death, neuroinflammation, and gliosis, which can be easily assessed for drug screening.

Trending Products

Creative Biolabs is passionate about preparing organotypic brain slices from various brain regions: hippocampus, cortex, cerebellum, thalamus, hypothalamus, striatum, and substantia nigra. Whole coronal or sagittal slices also can be offered according to your specific needs. Normally, these slice cultures are obtained from postnatal wide-type mice or rats. Organotypic brain slices from adult animals and transgenic animals such as 3xTg-AD mice can be customized.

Cat. No.	Product Name	Species
NRYF-0124-HZ1466	NeuroBiologics™ Organotypic Hippocampal Brain Slices of C57BL/6 Mouse	C57BL/6 Mouse
NRYF-0124-HZ1471	NeuroBiologics™ Organotypic Cortical Brain Slices of C57BL/6 Mouse	C57BL/6 Mouse
NRYF-0124-HZ1476	NeuroBiologics™ Organotypic Cerebellar Brain Slices of C57BL/6 Mouse	C57BL/6 Mouse
NRYF-0124-HZ1481	NeuroBiologics™ Organotypic Thalamic Brain Slices of C57BL/6 Mouse	C57BL/6 Mouse

For Research Use Only. Not For Clinical Use.

Related Products

NeuroBiologics™ Organotypic Thalamic Brain Slices of WS Rat [CAT#: NRYF-0124-HZ1484]

Strain:: Wistar Rat

Species:: Rat

Tissue:: Brain

Tissue Site:: Thalamus

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