NeuroMab™ Mouse Anti-CD68 Monoclonal Antibody (CBP1566)

Product Overview

Description

The mouse anti-rat CD68 clone ED1 recognizes the rat ED1 antigen, a strongly glycosylated protein of about 90-110 KDa, also known as rat CD68. The ED1 antigen is expressed on most macrophage populations and monocytes, and is considered a pan-macrophage marker in rats. ED1 is mainly expressed on the lysosomal membrane and slightly expressed on the cell surface. The expression of ED1 antigen is mainly cytoplasmic, and the results of flow cytometry can be improved by using membrane permeabilization procedures (such as Leucoperm) before staining.

Immunogen

Rat spleen cells.

Species Reactivity

Rat; Bovine

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1566

Applications

Relevant Diseases

Alzheimer's Disease

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1358

Product Properties

Form

Liquid

Formulation

PBS
0.09% Sodium Azide
1% BSA

Concentration

0.05 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Target

CD68

Official Name

CD68

Full Name

CD68 Molecule

Alternative Names

CD68 Molecule; CD68 Antigen; Scavenger Receptor Class D, Member 1; Macrophage Antigen CD68; GP110; Macrosialin; SCARD1; LAMP4

Gene ID

968(Human); 12514(Mouse); 287435(Rat)

Uniprot ID

P34810(Human); P31996(Mouse)

References

1. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
2. Damoiseaux, J.G. et al. (1994) Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1.
3. Bauer, J. et al. (1994) Phagocytic activity of macrophages and microglial cells during the course of acute and chronic relapsing experimental autoimmune encephalomyelitis.
4. Wu, L. et al. (2004) Dietary approach to attenuate oxidative stress, hypertension, and inflammation in the cardiovascular system.
5. Bao, F. et al. (2004) Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spinal cord injury in rats.
6. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.

Publications