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Creative Biolabs
Product

Human EPHB2 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP101]

Human EPHB2 knockout cell lysate

Species:
Human
Cell Types:
HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human EPHB2 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Pathways

Intracellular Signaling

Marker

Tyrosine Kinase Receptors

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

EPHB2

Official Name

EPHB2

Full Name

Ephrin type-B receptor 2

Alternative Names

EPHB2; Ephb2; Cek5; Drt; ETECK; Erk; Hek5; Nuk; Prkm5; Qek5; Sek3; Tyro5; CAPB; EK5; EPHT3; PCBC; EPH receptor B2; DRT; ERK; BDPLT22

Gene ID

2048(Human); 13844(Mouse); 313633(Rat)

Uniprot ID

P29323(Human); P54763(Mouse); B2B9B0(Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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