Creative Biolabs

Human SYP Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP56]

Human SYP knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HEK 293T

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human SYP has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HEK 293T

Cell Location

Epithelial

Applications

Cell Assay

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon2.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Relevant Diseases

Neurological Disorders

Research Areas

Neurotransmission

Pathways

Secretory Signaling

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

Synaptophysin

Official Name

SYP

Alternative Names

SYP; MRX96; MRXsynaptophysin

Gene ID

6855(Human); 20977(Mouse); 24804(Rat)

Uniprot ID

P08247(Human); Q62277(Mouse); P07825(Rat)
For Research Use Only. Not For Clinical Use.
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