Creative Biolabs

Human SNAP29 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP54]

Human SNAP29 knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HeLa

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Description
This is a HeLa cell transfected lysate in which Human SNAP29 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 5 bp deletion in exon1.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neurotransmission
Pathways
Secretory Signaling
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
SNAP29
Official Name
SNAP29
Alternative Names
Synaptosome Associated Protein 29; Soluble 29 KDa NSF Attachment Protein; Vesicle-Membrane Fusion Protein SNAP-29; Synaptosomal-Associated Protein; 29kDa; SNAP-29; Synaptosomal-Associated Protein; 29kD; Synaptosome Associated Protein 29kDa; Synaptosomal-Associated Protein 29; CEDNIK;
Gene ID
9342(Human); 67474(Mouse); 116500(Rat)
Uniprot ID
O95721(Human); Q9ERB0(Mouse); Q9Z2P6(Rat)
For Research Use Only. Not For Clinical Use.
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