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Creative Biolabs
Product

Human FLOT2 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP61]

Human FLOT2 knockout cell lysate

Species:
Human
Cell Types:
Other Cells

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human FLOT2 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon4.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Neurotransmission

Pathways

Secretory Signaling

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

Flotillin 2

Official Name

FLOT2

Full Name

Flotillin-2

Alternative Names

FLOT2; ECS-1; ECS1; ESA; ESA1; M17S1; flotillin 2

Gene ID

2319(Human); 14252(Mouse); 83764(Rat)

Uniprot ID

Q14254(Human); Q60634(Mouse); Q9Z2S9(Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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