Creative Biolabs

Human FLOT2 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP61]

Human FLOT2 knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HEK 293T

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Bulk Quote
Description
This is a HEK293T cell transfected lysate in which Human FLOT2 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HEK 293T
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon4.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neurotransmission
Pathways
Secretory Signaling
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12
Tissue Source
Kidney
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Flotillin 2
Official Name
FLOT2
Full Name
Flotillin-2
Alternative Names
FLOT2; ECS-1; ECS1; ESA; ESA1; M17S1; flotillin 2
Gene ID
2319(Human); 14252(Mouse); 83764(Rat)
Uniprot ID
Q14254(Human); Q60634(Mouse); Q9Z2S9(Rat)
For Research Use Only. Not For Clinical Use.
Send Inquiry Send Inquiry
Inquiry Basket
compare

Go to compare