Creative Biolabs

Human Neural Stem Cell Line

[CAT#: NCL-2103-P83]

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Stem Cell

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Description
Neural stem cells (NSC) are neural progenitor cells that can differentiate into different types of cells in the nervous system under defined conditions. Utilizing their continuous reproduction, NSC has great potential in neurogenesis, neurodegenerative diseases and clinical transplantation applications. Creative Biolabs' human neural stem cell line is derived from human induced pluripotent stem cells, which are generated from adult skin fibroblasts using Creative Biolabs' unique pollution-free free iPSC reprogramming method. When cultured in a medium containing bFGF and EGF, these cells can proliferate regularly without differentiation into neural lineage cells.
Cell Types
Neural Stem Cell
Applications
Cell Assay
Species
Human
Size
1x10^6 cells/vial
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry Ice - Overnight Shipping
Storage
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each lot of hNSCs is tested for growth and viability after recovery from cryopreservation. In addition, each lot is tested for expression of Nestin to ensure its undifferentiating characteristics.
For Research Use Only. Not For Clinical Use.
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