iNeu™ Human Neural Stem Cells - Alzheimer's Disease Patient (APOE4 HOM)

Description

Creative Biolabs human iPSC-derived neural stem cells (NSC) are derived from integration-free, induced pluripotent stem cells (iPSC) under fully defined neural induction conditions. These NSCs express typical markers of cerebral cortex neural stem and progenitor cells, such as PAX6 , FOXG1 and Nestin, and when they are laid in a single layer, they will spontaneously form a polarized neural tube rosette structure. In addition, Creative Biolabs NSC can produce a series of cerebral cortex excitatory and inhibitory neurons, these neurons have electrical activity And has the ability to form functional synapses and circuits in vitro. After thawing and plating, neural stem cells finally differentiate to obtain mature electrophysiological properties, and form a functional synaptic network within 40 to 50 days.

Following our protocols and using our tailor-made media and reagent packs, Creative Biolabs NSC can be easily distinguished into neurons or mixed neuronal cell types. After synchronous differentiation, highly purified neurons can be produced from Creative Biolabs NSC. Using our special coating reagent, it is possible to culture neurons from Creative Biolabs NSC for a long period of time (> 1 year). NSC can be obtained from multiple donors to meet your research needs and has a wide range of characteristics.

Cell Types

Neural Stem Cell

Applications

Cell Assay

Mutation Description

APOE4 homozygous

Species

Human

Size

1.5 million cells & Neural Plating-XF Medium

Growth Pattern

Adherent

Tissue Source

Fibroblasts (87 Yr Female)

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

vapour phase nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.

Additional Donor Information

The donor (Caucasian) had autopsy-confirmed Alzheimer's Disease with an onset of symptoms after age 70. The donor's parent and 3 siblings were also affected. The donor was homozygous for the 4 allele of apolipoprotein E.