NeuroMab™ Anti-CD183 BBB Shuttle Antibody(NRZP-1022-ZP3427)

Figure 1 illustrates the inhibition of IP-10-induced Th1 cell migration by murine anti-CXCR3 mAb. Ab #1-5D4A; Ab #2-8A5A; Antibody #3-19G2; Antibody #4-V36E5A; Antibody #5-V44D7A; Ab#6-37B5A; 9-V3G6A; Antibody #10-23E12A.

Figure 2 illustrates the inhibition of chemokine binding to CXCR3 by murine anti-CXCR3 mAb and humanized anti-CXCR3 mAb.

Figure 3 illustrates inhibition of chemokine-mediated chemotaxis by murine anti-CXCR3 mAb and humanized anti-CXCR3 mAb.

Figure 4 illustrates inhibition of mouse CXCR3 mAb binding to CXCR3+ NSO cells by commercial CXCR3 mAb.

CXCR3-transfected NSO cells were incubated with approximately 0.5 nM Eu-CXCR3 mAb in the presence of various concentrations of unlabeled commercial CXCRmAb. A dose-dependent inhibition of Eu-CXCR3 mAb binding to CXCR3+ NSO cells was observed.

Figure 5 illustrates the125I-CXCL10 binding assay.

Th1 cells were incubated with 125I-CXCL10 in 96-well plates in the presence or absence of various concentrations of CXCRmAb. Cell-bound 125I-CXCL10 was separated from free radioactivity by an oil column and counted using a gamma counter. IC50 values were calculated using Prizm software. Leading candidates are highlighted in green.

Figure 6 illustrates the binding of Eu-CXCR3 mAb to Th1 cells.

Figure 7 illustrates the inhibitory effect of humanized CXCR3Ab on Eu-CXCR3 mAb. Ton

Th1 cells were incubated with Eu-CXCR3 mAb in 96-well plates in the presence or absence of various concentrations of humanized CXCRmAb.

Figure 8 illustrates the inhibition of Eu-CXC10 by humanized CXCR3Ab.

Th1 cells were incubated with Eu-CXCL10 in 96-well plates in the presence or absence of various concentrations of humanized CXCRmAb.

Figure 9 illustrates that humanized CXCR3Ab inhibits CXCL10-induced chemotaxis of Th1 cells.

Chemotaxis assay was performed in a ChemoTx 96-well plate (Neuro Probe, Inc). Approximately 29 μL of CXCL10 or buffer control was added to the bottom wells. 25 μL of Th1 cell suspension in the absence or presence of various concentrations of humanized antibodies was added directly on the wells of the filter. After 2 hr incubation at 37° C., cells migrated to the bottom wells were determined by cell titer glo method (Promega).