NeuroMab™ Anti-Lingo1 Antibody(NRP-0422-P1660)

Product Overview

Description

The antibody has been purified from phage display and shown to bind specifically to LINGO-1 by both ELISA and FACS. The antibody promotes the differentiation of human oligodendrocytes, promotes remyelination in brains treated with lysolecithin, and promotes in vivo myelination in the lysolecithin assay.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Applications

WB; IHC; ELISA; FC; In Vitro; In Vivo

Relevant Diseases

Multiple Sclerosis

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

Lingo1

Official Name

LINGO1

Full Name

Leucine rich repeat and Immunoglobin-like domain-containing protein 1

Alternative Names

LINGO1; LERN1; LRRN6A; UNQ201; leucine rich repeat and Ig domain containing 1; MRT64

Gene ID

84894(Human); 235402(Mouse); 315691(Rat)

Uniprot ID

Q96FE5(Human); Q9D1T0(Mouse)

Product Pictures

ELISA

Figure 1 is a graph showing Li113 Fab binding to LINGO-1 measured by ELISA assay.

FuncS

Figure 2 is a graph showing the effectiveness of LINGO-1 monoclonal antibody and Fab in an oligodendrocyte differentiation assay. Bar heights represent MBP concentrations measured by ELISA. Antibodies were tested at concentrations of 1 μg/mL (black), 0.3 μg/mL (dark gray), 0.1 μg/mL (light gray), and 0.03 μg/mL (white).

FuncS

Figure 3 Anti-LINGO-1 antibody promotes myelination in vivo in a lysolecithin assay.

Anti-LINGO-1 antibody promotes in vivo myelination in the lysolecithin assay

ELISA

Figure 4 LINGO-1 ELISA assay showed that Li113 Fab bound to LINGO-1 with an EC50 of 0.09 nM. In the same experiment, the measured EC50 values of Li33 Fab, Li62 Fab and Li81 Fab were 0.30 nM, 0.26 nM and 0.11 nM, respectively.

ELISA

Figure 5 The aggregation state of Li33 Ig2 was further assessed by analytical ultracentrifugation.

FCM

Figure 6 shows that PEGylated Li81 and Li113 can bind to LINGO-1.

ELISA

Figure 7 Determination of the efficacy of Li81 antibody and its fragments by ELISA assay to determine LINGO-1 binding.

ELISA

Figure 8 Determination of the efficacy of Li81 antibody and its fragments by oligodendrocyte proliferation assay ("OPC assay") to determine LINGO-1 binding.