NeuroMab™ Anti-LINGO-1 BBB Shuttle Antibody(NRZP-1022-ZP3053)

Figure 1 is a graph showing Li113 Fab binding to LINGO-1 measured by ELISA assay.

Figure 2 is a graph showing the effectiveness of LINGO-1 monoclonal antibody and Fab in an oligodendrocyte differentiation assay. Bar heights represent MBP concentrations measured by ELISA. Antibodies were tested at concentrations of 1 μg/mL (black), 0.3 μg/mL (dark gray), 0.1 μg/mL (light gray), and 0.03 μg/mL (white).

Figure 3 Anti-LINGO-1 antibody promotes myelination in vivo in a lysolecithin assay.

Anti-LINGO-1 antibody promotes in vivo myelination in the lysolecithin assay

Figure 4 LINGO-1 ELISA assay showed that Li113 Fab bound to LINGO-1 with an EC50 of 0.09 nM. In the same experiment, the measured EC50 values of Li33 Fab, Li62 Fab and Li81 Fab were 0.30 nM, 0.26 nM and 0.11 nM, respectively.

Figure 5 The aggregation state of Li33 Ig2 was further assessed by analytical ultracentrifugation.

Figure 6 shows that PEGylated Li81 and Li113 can bind to LINGO-1.

Figure 7 Determination of the efficacy of Li81 antibody and its fragments by ELISA assay to determine LINGO-1 binding.

Figure 8 Determination of the efficacy of Li81 antibody and its fragments by oligodendrocyte proliferation assay ("OPC assay") to determine LINGO-1 binding.

Figure 9 Efficacy of Li81 Antibody and Its Fragments LINGO-1 Binding Determined by Rat Remyelination Assay.