NeuroMab™ Anti-Slc18a2/VMAT2 BBB Shuttle Antibody, Clone N449/73

Product Overview

Description

The BBB is a highly selective membrane that surrounds the blood vessels in the brain, and it can prevent many molecules, including potentially life-saving drugs, from entering the brain. The anti-VMAT2 BBB Shuttle Antibody is designed to bind to receptors on the surface of cells in the BBB, and once bound, the antibody can cross the BBB and into the brain. This technology has the potential to revolutionize the treatment of brain disorders by enabling drugs to reach the brain more effectively.

Immunogen

Fusion protein amino acids 42-132 (first lumenal domain) of mouse Slc18a2 (also known as Synaptic vesicular amine transporter, Monoamine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, VAT2, Svmt, Svat and Vmat2, accession number Q66L40)
Rat: 82% identity (75/92 amino acids identical)
Human: 73% identity (67/92 amino acids identical)
<30% identity with Slc18a1/VMAT1

Construction

We offer several options for BBB shuttle antibody design. Antibody construction can be customized according to customer requirements.

Species Reactivity

Rat; Mouse; Human

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

N449/73

Applications

WB; IHC; ICC

Application Notes

Does not cross-react with Slc18a1/VMAT1 or Slc18a3/VAChT

Research Areas

Transporters

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

VMAT2

Official Name

SLC18A2

Full Name

VMAT2

Alternative Names

SLC18A2; SVAT; SVMT; VAT2; VMAT2; solute carrier family 18 member A2; Vesicular monoamine transporter 2; PKDYS2

Uniprot ID

Q66L40

Product Pictures

Fig. 1 Immunoblot against extracts of COS cells transiently transfected with Myc/Flag-tagged VMAT1, VMAT2, VAChT or Kv2.1 plasmid probed with N449/73 (left) or rabbit anti-Flag (right).

Fig. 2 immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N449/73 (left) and N52A/42 (right) TC supe.