NeuroMab™ Rat Anti-Mouse CD45 Monoclonal Antibody (CBP2342)

Product Overview

Description

CD45RB is a 220 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.

Immunogen

anti-Ig activated murine B cells

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a, κ

Clone Number

CBP2342

Applications

Application Notes

FCM (≤0.5 µg per million cells in 100 µl volumel)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1729

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD45

Official Name

PTPRC

Full Name

Protein tyrosine phosphatase, receptor type, C

Alternative Names

CD45RB

Gene ID

5788 (Human); 19264 (Mouse)

Uniprot ID

P08575 (Human); P08575 (Mouse)

References

1. Lee KM, et al. 2014. Cell Transplant. 23:51.
2. Kamanaka M, et al. 2011. J. Exp. Med. 208:1027.
3. Gagliani N, et al. 2011. PLoS One 6(12):e28434.
4. Ten Hove T, et al. 2004. J. Leuko. Biol. 75:1010.
5. Ogilvy S, et al. 2003. J. Immunol. 171:1792.

Publications