Mouse Anti-PTPRC Monoclonal Antibody (CBP2656)

Product Overview

Description

CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity.

Expression Host

Mammalian cells

Immunogen

CD45 fusion protein Ag13712

Species Reactivity

Human; Porcine; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP2656

Applications

FC; IF; IHC; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Metabolism

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1667 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD45

Official Name

PTPRC

Full Name

Protein tyrosine phosphatase, receptor type, C

Alternative Names

B220; CD45; CD45R; GP180; L CA; LCA; Leukocyte common antigen; LY5; PTPRC; RPTPC; T200

Gene ID

5788(Human)

Uniprot ID

P08575(Human)

References

1. Li, Q., Zhai, Y., Man, X., Zhang, S., & An, X. (2020). Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells. Cellular Reprogramming, 22(2), 82-89. 2. Matsunari, H., Watanabe, M., Hasegawa, K., Uchikura, A., Nakano, K., Umeyama, K.,... & Nishinakamura, R. (2020). Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation. Stem cell reports, 14(1), 21-33.

Publications