Mouse Anti-rodent Ki67 Monoclonal Antibody (CBP6655)
[CAT#: NAB2007FY1516]
Mouse Monoclonal to rodent Ki67
- Host Species:
- Mouse
- Species Reactivity:
- Rat; Mouse
- Applications:
- WB; IF; IHC
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
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Description
The Ki67 protein was first discovered when researchers tried to produce cancer cell-specific monoclonal antibodies by injecting nuclear preparations of Hodgkin's lymphoma cells into mice (1). They obtained a monoclonal antibody that can recognize two large proteins with apparent molecular weights of 345kDa and 395kDa. The clone was named Ki67 after the initial work was completed in Kiel, Germany, and the 96-well plate where the clone was found. It was found that these two proteins are expressed in large amounts in proliferating cells, but not in resting cells. Later work showed that they are the products of a single gene. The presence of Ki67 protein is often used as an indicator of cell proliferation, and its expression level is one of the most reliable biomarkers of cancer cell proliferation status (2-5).
Immunogen
A recombinant construct containing amino acids 956-1322 of the mouse sequence XP_006507475, expressed in E. coli and purified from E. coli.
Species Reactivity
Rat; Mouse
Clonality
Monoclonal
Host Species
Mouse
Isotype
IgG1
Clone Number
CBP6655
Applications
WB; IF; IHC
Application Notes
WB: 1:5000-10000
IF 1:2000-5000 IHC 1:1000
IF 1:2000-5000 IHC 1:1000
Product Properties
Form
Liquid
Formulation
50% PBS, 50% glycerol
Preservatives
5mM NaN3
Concentration
1 mg/ml
Shipping
Gel Pack
Storage
Storage for short term at 4°C recommended, for longer term at -20°C, minimize freeze/thaw cycles
Handling Advice
The antibody solution should be gently mixed before use.
Research Use Only
For research use only
Target
Publications
Publications (0)
Neural Antibodies
Kits
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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