PRV-CMV-RFP

[CAT#: NTA-2011-ZP12] Review(5) Q&As(3)

Retrograde, multisynaptic

Tracer Type:: Retrograde tracing virus

Applications:: Neural Tracing

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Product Overview

Description

Pseudorabies virus (PRV) has a double-stranded DNA genome with a genome of approximately 150 kb, which encodes capsid, outer skin, and envelope proteins. The reverse-transcribed anti-synaptic PRV is modified from the vaccine strain Bartha and can be used to analyze the connection structure of the brain center and peripheral neurons. After the virus infects nerve cells, it can replicate in the cell and express the target gene. The offspring virus is transported to the synapse, and then retrogrades the counter-synaptic label of the upstream neuron for further replication and transmission across the synapse.

After years of research, Creative Biolabs has established a stable production platform and can provide pseudorabies virus (PRV) services to promote PRV-based neuron tracking research.

Application field
●Multi-synaptic tracking from the periphery to the center of the brain or from higher to lower brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.

Tracer Type

Retrograde tracing virus

Functional Gene

eArchT3.0

Virus Type

Pseudorabies Virus (PRV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Direction of Transport

Retrograde

Promoter

CMV

Expression

Normal Expression

Properites

Storage

Please refer to the protocal.

Research Use Only

For research use only.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The results have been reproducible, and our research has greatly benefited from this product

The RFP expression is bright and distinct, which greatly simplifies the identification of infected neurons. We also appreciate the efficiency of viral transduction; it’s consistent and reliable across various experiments. Moreover, the product comes with comprehensive instructions, making it user-friendly even for those new to neural tracing techniques.

Excellent

PRV-CMV-RFP has significantly improved our neural tracing experiments

RFP provides clear and vivid labeling of neurons, making it easy to trace neural connections. The virus's tropism and specificity are also commendable, ensuring precise targeting of the neurons we aim to study. Additionally, the stability and longevity of the fluorescence allow for extended observation periods. The overall quality of the product is excellent, and we will continue to use it for our neural circuit mapping projects.

Excellent

Highly recommended for neural tracing studies

The fluorescence is stable and persists for the duration of our experiments, allowing for detailed analysis of neural networks. The product's efficiency and reliability have significantly enhanced our research capabilities. We also appreciate the support provided by the supplier in addressing any queries we had during the initial setup.

Excellent

It’s an indispensable tool for our neuroscience research

This product has allowed us to visualize neural circuits with remarkable clarity. Additionally, the shipment was prompt, and the product arrived in excellent condition.

Excellent

Our lab has seen fantastic results using PRV-CMV-RFP for neural tracing

The virus shows high infectivity and specificity, ensuring precise labeling of neurons. Moreover, the stability of the fluorescent signal over time has been impressive, enabling us to conduct long-term studies.

Q&As

Can PRV-CMV-RFP be used in a variety of animal models?

PRV-CMV-RFP is versatile and can be used in various animal models, including rodents and non-human primates. The choice of model depends on the research question and the specific neural circuits being studied. Always verify that the animal model is compatible with the virus and that the experimental conditions align with the recommendations.

What are the expected results when using PRV-CMV-RFP for neural tracing?

When PRV-CMV-RFP is successfully used, you should observe red fluorescence in neurons that were infected by the virus. This fluorescence will highlight the pathways and connections between neurons, allowing for detailed mapping of neural circuits. The extent and pattern of fluorescence provide insights into the connectivity and organization of the neural networks being studied.

What troubleshooting steps should be taken if PRV-CMV-RFP does not produce expected results?

If the expected results are not observed, first check the virus preparation and administration procedures to ensure proper handling and delivery. Verify that the imaging equipment is properly calibrated for RFP detection. Additionally, review the experimental conditions and animal model compatibility. If problems persist, consult for technical support and consider troubleshooting steps provided in the product manual.