Product
Aβ1-40 Induced Neurotoxicity Assay Cell Line
[CAT#: NCL2110P255]
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: Rat Primary Neurons
Relevant Diseases
Alzheimer's Disease; CNS
Assay
Aβ1-40 Induced Neurotoxicity Assay
Assay Description
Beta-amyloid (1-40) neuron poisoning is a useful in vitro model of Alzheimer's disease (AD). This model allows evaluation of different drugs to inhibit the nerves in AD based on the accumulation of neurotoxic amyloid beta peptides The ability of meta-injury. Aβ1-40-induced neurotoxicity assay has the advantage of rapid screening of molecular neuroprotective potential.
In our assay, we incubate rat primary neurons in parallel with the test compound and Aβ1-40 peptide. After 24 hours of incubation, we analyzed different parameters related to all stages of the cell death process. Some of the endpoints we analyzed were cell viability, LDH release, neurite outgrowth, or caspase activation.
In our assay, we incubate rat primary neurons in parallel with the test compound and Aβ1-40 peptide. After 24 hours of incubation, we analyzed different parameters related to all stages of the cell death process. Some of the endpoints we analyzed were cell viability, LDH release, neurite outgrowth, or caspase activation.
Assay Characteristics
Readout: Neurite Outgrowth, LDH Release, Caspase Activation, Cell Viability
Damage Induction: Aβ1-40 peptide
Type of Assay: Cell-based / Neuroprotection
Damage Induction: Aβ1-40 peptide
Type of Assay: Cell-based / Neuroprotection
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
Amyloid Beta 1-40
Full Name
Aβ1-40
Alternative Names
APP; Aβ; Abeta; Amyloid β
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
