Aβ1-42 Induced Neurotoxicity Assay Cell Line

[CAT#: NCL2110P256]

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: Rat Primary Neurons

Neurological Disease Models

AD-related Cells

Cell Types

Other Cells

Relevant Diseases

Alzheimer's Disease; CNS

Assay

Aβ1-42 Induced Neurotoxicity Assay

Assay Description

Beta-amyloid (1-42) oligomers are a useful in vitro model of Alzheimer's disease (AD) for neuronal poisoning. This model allows assessment of differences based on the accumulation of neurotoxic amyloid beta The ability of drugs to inhibit neuronal damage in AD is oligomers. The neurotoxicity assay induced by Aβ1-42 has the advantage of rapid screening of molecular neuroprotective potential.

In our assay, we incubate rat primary neurons in parallel with the test compound and Aβ1-42 peptide. After 24 hours of incubation, we analyzed different parameters related to all stages of the cell death process. Some of the endpoints we analyzed were cell viability, LDH release, neurite outgrowth, or caspase activation.

Assay Characteristics

Readout: Neurite Outgrowth, LDH Release, Caspase Activation, Cell Viability

Damage Induction: Aβ1-42 Oligomers

Type of Assay: Cell-based / Neuroprotection

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.