Amyloid-beta peptide Oligomerization Assay Cell Line

[CAT#: NCL2110P246]

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: Rat primary Neurons, Immortalized Human Microglia or SH-SY5Y cell line

Neurological Disease Models

AD-related Cells

Cell Types

Other Cells

Relevant Diseases

Alzheimer's Disease; CNS

Assay

Amyloid-beta peptide Oligomerization Assay

Assay Description

The amyloid-β peptide oligomerization assay is a functional cell-based assay used to identify inhibitors of the amyloid peptide oligomerization process. We incubate rat primary neurons, human microglia or SH-SY5Y cells with the test compound and Aβ1-42 peptide in parallel within 24 hours. After that, we measured the Aβ1-42 oligomerization process and analyzed the number of thiazine staining oligomers related to the number of cells.

Assay Characteristics

Readout: Stained oligomers quantification

Agonist: Aβ1-42 peptide (25 μM)

Assay Type: Cell-based

Detection Method: Image Analysis

Positive Compound: Galantamine (3 μM)

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.