Amyloid-beta peptide Oligomerization Assay Cell Line
[CAT#: NCL2110P246]
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: Rat primary Neurons, Immortalized Human Microglia or SH-SY5Y cell line
Neurological Disease Models
AD-related Cells
Cell Types
Other Cells
Relevant Diseases
Alzheimer's Disease; CNS
Assay
Amyloid-beta peptide Oligomerization Assay
Assay Description
The amyloid-β peptide oligomerization assay is a functional cell-based assay used to identify inhibitors of the amyloid peptide oligomerization process. We incubate rat primary neurons, human microglia or SH-SY5Y cells with the test compound and Aβ1-42 peptide in parallel within 24 hours. After that, we measured the Aβ1-42 oligomerization process and analyzed the number of thiazine staining oligomers related to the number of cells.
Assay Characteristics
Readout: Stained oligomers quantification
Agonist: Aβ1-42 peptide (25 μM)
Assay Type: Cell-based
Detection Method: Image Analysis
Positive Compound: Galantamine (3 μM)
Agonist: Aβ1-42 peptide (25 μM)
Assay Type: Cell-based
Detection Method: Image Analysis
Positive Compound: Galantamine (3 μM)
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
Amyloid beta
Full Name
Amyloid-Beta Peptide
Alternative Names
APP; Aβ; Abeta; Amyloid β
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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