NeuroMab™ Anti-Amyloid Beta BBB Shuttle Antibody(NRZP-1022-ZP2565)

Fig.1 is a graph depicting binding of Aβ to chimeric 3D6 (PK1614) compared to mouse 3D6.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates from PDAPP mouse brain tissue. IgG was used as a negative control in this experiment because it is unable to bind Aβ and therefore cannot induce phagocytosis.

Fig.2 is a graph depicting the competition of biotinylated 3D6 against unlabeled 3D6, PK1614 and 10D5 for binding to Aβ.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates from PDAPP mouse brain tissue. IgG was used as a negative control in this experiment because it is unable to bind Aβ and therefore cannot induce phagocytosis.

Fig.3 depicts the results of an ELISA that measures the binding of humanized 3D6v1 and chimeric 3D6 to aggregated Aβ.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates from PDAPP mouse brain tissue. IgG was used as a negative control in this experiment because it is unable to bind Aβ and therefore cannot induce phagocytosis.

Fig.4 Immunohistochemistry on brain sections of PDAPP demonstrates the specificity of the h3D6v1 antibody.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates from PDAPP mouse brain tissue. IgG was used as a negative control in this experiment because it is unable to bind Aβ and therefore cannot induce phagocytosis.

Fig.5 Competitive binding analysis of h3D6.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates from PDAPP mouse brain tissue. IgG was used as a negative control in this experiment because it is unable to bind Aβ and therefore cannot induce phagocytosis.

Fig.6 Ex vivo assay using h3D6v2 antibody. The ability of h3D6v2 to stimulate microglia was tested by ex vivo phagocytosis assay.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates from PDAPP mouse brain tissue. IgG was used as a negative control in this experiment because it is unable to bind Aβ and therefore cannot induce phagocytosis.