NeuroMab™ Anti-Amyloid Beta BBB Shuttle Antibody(NRZP-1022-ZP2563)

Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human; Cynomolgus Monkey

Clonality

Monoclonal

Host Species

Humanized

Applications

ELISA; FC; IHC; In Vitro; In Vivo

Relevant Diseases

Alzheimer's Disease

Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only

Target

AmyloidBeta

Official Name

Amyloid Beta

Full Name

Amyloid Beta

Alternative Names

APP; Aβ; Abeta; Amyloid β

Product Pictures

ELISA

Fig.1 is a graph depicting binding of Aβ to chimeric 3D6 (PK1614) compared to mouse 3D6.

sAPPβ/sAPPα ratio over time.

ELISA

Fig.2 is a graph depicting the competition of biotinylated 3D6 against unlabeled 3D6, PK1614 and 10D5 for binding to Aβ.

sAPPβ/sAPPα ratio over time.

ELISA

Fig.3 depicts the results of an ELISA that measures the binding of humanized 3D6v1 and chimeric 3D6 to aggregated Aβ.

sAPPβ/sAPPα ratio over time.

IHC

Fig.4 Immunohistochemistry on brain sections of PDAPP demonstrates the specificity of the h3D6v1 antibody.

sAPPβ/sAPPα ratio over time.

ELISA

Fig.5 Competitive binding analysis of h3D6.

sAPPβ/sAPPα ratio over time.

FuncS

Fig.6 Ex vivo assay using h3D6v2 antibody. The ability of h3D6v2 to stimulate microglia was tested by ex vivo phagocytosis assay.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates from PDAPP mouse brain tissue. IgG was used as a negative control in this experiment because it is unable to bind Aβ and therefore cannot induce phagocytosis.

Publications