NeuroMab™ Anti-Tau; pTau Antibody(NRP-0422-P1721)

Product Overview

Description

The antibody reacted with phosphorylated and non-phosphorylated tau showing that their recognition epitopes are phosphorylation independent.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Applications

WB; IHC; ICC; In Vitro; In Vivo

Relevant Diseases

Alzheimer's Disease

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

TaupTau

Alternative Names

MAPT; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; microtubule associated protein tau; Tau proteins; tau-40; phosphorylated tau

Gene ID

4137(Human); 17762(Mouse); 29477(Rat)

Uniprot ID

P10636(Human); P10637(Mouse); P19332(Rat)

Product Pictures

Figure 1 shows the characterization of the specificity of the tau antibody by immunoblot analysis of total brain lysates from nTg, tau KO, and PS19 tau Tg mice using actin as a control.

Figure 2 shows the specificity of tai antibodies raised against the tau peptide p193-211, as determined by immunoblotting with recombinant tau protein phosphorylated in vitro by p42 MAPK or GSK3β.

IHC

Figure 3 shows immunocytochemistry of representative tau pathology in human AD brain and JNPL3 Tg mice with antibodies 2D1 and 7F2.

Immuno-reactivity of previously characterized phospho-tau antibodies AT8 and new tau antibodies 2D1 or 7F2 in the hippocampus of a control subject or a subject with AD, and in the spinal cord of 12 month old nTg and JNPL3 Tg mice. Arrows indicating NFTs in human brain or NFT-like inclusion pathology in JNPL3 mice. Asterisks depict dystrophic neurites within senile plaques. Bar=100 μm, and 200 μm for insets.