NeuroMab™ Mouse Anti-GAD65 Monoclonal Antibody (CBP2353)

Description

GAD gene encodes one of the several forms of glutamic acid decarboxylase, which is identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid (GABA) from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. GAD65 targets presynaptic clusters in axons and exhibits relative dendritic exclusion. GAD65 synthesizes GABA for neurotransmission and is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter (VGAT), which, as a complex, helps package GABA into vesicles for release during neurotransmission.

Immunogen

Peptide immunogen corresponding to amino acids 4-22 of human GAD65.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2353

Applications

WB; IHC-P; IP

Application Notes

WB (1.0-10 µg/mL); IHC-P (1.0-5 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1737

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

GAD65

Official Name

GAD65

Full Name

Glutamate Decarboxylase 65

Alternative Names

Glutamate Decarboxylase 2; GAD65; Glutamate Decarboxylase 65 kD Isoform; GABA

Gene ID

2571(Human); 14415(Mouse); 24379(Rat)

Uniprot ID

Q99259(Human); P48318(Mouse); P18088(Rat)

1. Walls A, et al. 2011. J. of Cerebral Blood Flow & Metabolism 31:494.