Mouse Anti-GAD2 Monoclonal Antibody (CBP6785)

Description

Synaptic signaling is the communication between cells that occurs at the synaptic level. Cells communicate with each other through direct contact (near secretory signals), short distances (paracrine signals), or long distances and scales (endocrine signals). Other cells form gap junctions, connecting their cytoplasm to The cytoplasm of adjacent cells. When the sending neuron is activated, the electrical impulse will propagate with it Axon. Once the impulse reaches the synapse, it triggers the release of neurotransmitters. The main excitatory neurotransmitter in the brain is glutamate (GLU). The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brain stem and spinal cord) are common Amino acids involved in multiple metabolic cycles. Glutamate decarboxylase or glutamate decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate into GABA and CO 2. GAD uses PLP as a cofactor. The reaction proceeds as follows:
HOOC-CH2-CH2-CH(NH2)-COOH→CO2 + HOOC-CH2-CH2-CH2NH2

In mammals, GAD exists in two isoforms with molecular weights of 67 and 65 kDa (GAD67 and GAD65), which are encoded by two different genes on different chromosomes (GAD1 and GAD2 genes, which are human 2 and Chromosome 10). GAD67 and GAD65 are expressed in the brain using GABA as a neurotransmitter, and are also expressed in insulin-producing beta cells of the pancreas, depending on the species. These two enzymes together maintain the main physiological supply of mammalian GABA, although it may also be caused by the enteric nervous system, brain, and other places through the action of diamines Synthesized from putrescine. Oxidase and aldehyde dehydrogenase 1a1.

Several truncated transcripts and peptides of GAD67 can be detected in the developing brain, but their function (if any) is unknown.

Epitope

Binds to an located within the peptide sequence AACACDQKPC

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP6785

Applications

IHC; WB

Application Notes

IHC: 1:50-1:200
WB: 1 µg/ml

Research Areas

Neurotransmitter

Form

Liquid

Formulation

PBS, pH 7.2, 40% glycerol

Preservatives

0.02% Sodium azide

Concentration

1 mg/ml

Purification

Protein A purified

Shipping

Gel Pack

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

GAD65

Official Name

GAD2

Full Name

glutamic acid decarboxylase (GAD)

Alternative Names

GAD2

Gene ID

2572(Human); 14417(Mouse); 24380(Rat)

Uniprot ID

Q05329(Human); P48320(Mouse); Q05683(Rat)