Mouse Anti-GAD2 Monoclonal Antibody (CBP1581)

Description

Mouse anti-human glutamate decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes human glutamate decarboxylase 2, also known as 65 kDa glutamate decarboxylase, 65 kDa glutamate decarboxylase or GAD -65. Glutamate decarboxylase 2 is a lipid-anchored membrane-associated protein of 585 amino acids ~ 65 kDa, responsible for catalyzing the production of γ-aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the central nervous system (Fenalti et al. 2007), expressing brain and pancreatic β cells in glutamate.
Glutamate decarboxylase 2 is a 65 kDa isoform of glutamate decarboxylase encoded by the GAD2 gene and mainly expressed by the nerve terminal, as opposed to the 67 kDa isoform (GAD67) encoded by the GAD1 gene mainly in the cell body. Autoantibodies against glutamate decarboxylase 2 have been detected in autoimmune diseases including Graves disease (Kallmann et al. 1997) and stiffness syndrome (Raju et al. 2005), and in patients with type I diabetes or high-risk populations Type I diabetes is the most common (Petersen et al., 1994). The N-terminal region of GAD65 is essential for targeting enzymes to secretory vesicles containing GABA (Shi et al., 1994).

Immunogen

Keyhole Limpet Haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65.

Species Reactivity

Human; Monkey; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1581

Applications

IHC-Fr; IHC-P; WB; IP; RIA

Application Notes

WB (1/100-1/1000)

Research Areas

Growth and Development

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

GAD65

Official Name

GAD2

Full Name

glutamic acid decarboxylase (GAD)

Alternative Names

GAD2

Gene ID

2572(Human); 14417(Mouse); 24380(Rat)

Uniprot ID

Q05329(Human); P48320(Mouse); Q05683(Rat)

1. Hampe, C.S. et al. (2001) A novel monoclonal antibody specific for the N-terminal end of GAD65.