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HOT Human Astrocytes

[CAT#: NCC20-9PZ01] Review(5) Q&As(3)

Cryopreserved ampule of Normal Human Astrocytes (NHA) containing ≥1 million cells

Species:
Human
Cell Types:
Astrocytes

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Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview

Description

Primary human astrocytes are isolated from the human brain and can be used to study the function of the central nervous system and how nerve cells interact. Astrocytes are glial cells found in the brain and spinal cord.

Cell Types

Astrocytes

Application Notes

• Guaranteed through 10 population doublings
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)

Relevant Diseases

Alzheimer's Disease; Parkinson's Disease

Research Areas

Neurotoxicity; Neurogenesis

Species

Human
Properties

Size

1 x Cryopreserved ampule of NHA containing ≥1 million cells

Form

Cryopreserved

Shipping

Dry Ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Quality Control

• Mycoplasma, bacteria, yeast and fungus test negative
• Not all donors and/or cell batches have detected HIV-1, hepatitis B and C
• Certificate of analysis is provided for each cell batch purchased
Donor Information

Donor Health

Normal
Publications

Publications (0)

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
The human astrocytes we ordered have been an incredible addition to our neuroscience research
The cells arrived in perfect condition, and their viability was outstanding. They responded well to the various treatments and assays we performed, showing robust and consistent results. This has significantly accelerated our project timeline.
user
Excellent
For our studies on astrocyte-neuron interactions
The cells were shipped quickly and in good condition. They maintained a healthy phenotype throughout our experiments, and the purity was impressive. We observed expected calcium signaling responses, which is critical for our work. Will definitely reorder for future projects.
user
Excellent
We purchased human astrocytes for a project on glioblastoma
They integrated well into our co-culture systems, and their proliferation rate was as advertised. The gene expression profiles matched well with primary astrocytes, giving us confidence in our experimental outcomes. Excellent product.
user
Excellent
I have been very pleased with the astrocytes from this supplier
They were delivered in excellent condition and have been easy to maintain in culture. The cells exhibit typical astrocytic properties, and their response to stress conditions has been consistent and reproducible.
user
Excellent
The astrocytes we received were of outstanding quality
We observed significant and reproducible cytokine release in response to injury models, which was crucial for our study. The technical support team was very helpful in optimizing our protocols.
Q&As
What is the recommended seeding density for culturing human astrocytes?
The recommended seeding density for human astrocytes varies depending on the experimental setup, but typically ranges from 5,000 to 10,000 cells per cm2. This ensures proper cell attachment and growth. Our technical support team can provide guidance based on specific experimental requirements, including advice on scaling up cultures for larger assays or specialized applications.
What quality control measures are in place for human astrocytes?
Our human astrocytes undergo extensive quality control measures, including viability assays, morphological assessments, and mycoplasma testing. Each batch is also tested for expression of astrocytic markers like GFAP. We include a certificate of analysis with every purchase, detailing the results of these tests to ensure that researchers receive high-quality, reliable cells for their experiments.
What types of assays are compatible with human astrocytes?
Human astrocytes can be used in various assays, including immunocytochemistry, calcium imaging, gene expression analysis, and neurotoxicity assays. They are suitable for high-throughput screening and drug discovery applications. Our support team can assist in selecting the appropriate assays and provide guidance on optimizing protocols to obtain reliable and reproducible results from your experiments.
For Research Use Only. Not For Clinical Use.
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