Human CYP1B1 Knockout A549 Cell Lysate

[CAT#: NCL2008ZP152]

Human CYP1B1 knockout cell lysate

Species:: Human

Cell Types:: A549 Cell

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Product Overview

Description

This is a A549 cell transfected lysate in which Human CYP1B1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

A549 Cell

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 37 bp deletion in exon2 and Insertion of the selection cassette in exon2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X,Y
D5S818: 11
D13S317: 11
D7S820: 8, 11
D16S539: 11, 12
vWA: 14
TH01: 8,9.3
TPOX: 8,11
CSF1PO: 10, 12

Tissue Source

Lung

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

CYP1B1

Official Name

CYP1B1

Full Name

Cytochrome P450 Family 1 Subfamily B Member 1

Alternative Names

Cytochrome P450 Family 1 Subfamily B Member 1; Cytochrome P450; Subfamily I (Dioxin-Inducible); Polypeptide 1 (Glaucoma 3; Primary Infantile); Cytochrome P450; Family 1; Subfamily B; Polypeptide 1; EC 1.14.14.1; CYPIB1; Flavoprotein-Linked Monooxygenase; Aryl Hydrocarbon Hydroxylase; Microsomal Monooxygenase;