Human GLUL Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP215]

Human GLUL knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human GLUL has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 8 bp deletion in exon 2.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Neurotransmitter

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8, 12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

GLUL

Official Name

GLUL

Full Name

Glutamate-Ammonia Ligase

Alternative Names

Glutamate-Ammonia Ligase ; Glutamine Synthetase ; Palmitoyltransferase GLUL ; EC 6..1.2 ; GLNS ; GS ; Glutamate-Ammonia Ligase (Glutamine Synthase); Cell Proliferation-Inducing Protein 59 ; Proliferation-Inducing Protein ; Glutamate--Ammonia Ligase ; Glutamate Decarboxylase ; Glutamine Synthase ; EC 2..1.225 ; PIG ; PIG59 ; GLUL

Publications