NeuroMab™ Anti-SCN9A Antibody(NRP-0422-P570)

Product Overview

Description

The antibody binds to a voltage sensor paddle (VSP) of Nav1.7, useful for treating pain, itch, neurogenic inflammation, or cough in a subject.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

ELISA; Block; Inhib; In Vitro; In Vivo

Relevant Diseases

Pain; Epilepsy

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

SCN9A

Official Name

SCN9A

Full Name

sodium voltage-gated channel alpha subunit 9

Alternative Names

SCN9A; ETHA; FEB3B; GEFSP7; HSAN2D; NE-NA; NENA; Nav1.7; PN1; SFNP; sodium voltage-gated channel alpha subunit 9

Gene ID

6335(Human); 20274(Mouse); 78956(Rat)

Uniprot ID

Q15858(Human); Q62205(Mouse); O08562(Rat)

Product Pictures

ELISA

Figure 1 shows the ELISA response of 1E16 mAb and 115 mAb using intact Nav1.7 DII VSD. Data are shown as mean SEM (n=3).

From HEK293 cells expressing human Nav1.7 channel (A), in the presence of 1E16 (100 nM) and peptide (1 μM) (B) and in the presence of 1E16 (100 nM) only (C).

ELISA

Figure 2 shows that 1E16 reduces inflammation and neuropathic pain and inhibits spinal cord synaptic transmission in mice.

Time course of licking and flinching behavior after plantar injection of 5% formalin.

ELISA

Figure 3 shows the effect of mAbs on sustained sodium currents (INaP) in small neurons from the entire dorsal root ganglion (DRG) of naive and nerve-injured mice (CCI.).

Time course of licking and flinching behavior after plantar injection of 5% formalin.

ELISA

Figure 4 shows the binding of 1E16 antibody to human Nav1.7. Data are shown as mean SEM (n=10).

Time course of licking and flinching behavior after plantar injection of 5% formalin.

ELISA

Figure 5 shows the state (usage) dependent inhibition of human Na 1.7 by 1E16.

Normalized current during a 30 ms depolarizing pulse from a holding potential of -120 mV to -10 mV in the presence of 100 nM 1E16 at 0.1 (A), 2 (0) and 10 (o) Hz Amplitude graph.

Publications