NeuroMab™ Rat Anti-Mouse CD40 Monoclonal Antibody (CBP2199)

Product Overview

Description

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Immunogen

CD 40 protein

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a, κ

Clone Number

CBP2199

Applications

FC; Block

Application Notes

FCM (≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1630

Product Properties

Form

Liquid

Formulation

0.2 µm filtered in PBS, pH 7.2, containing no preservative.

Concentration

1.0 mg/ml

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

<0.01 EU/µg of the protein

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD40

Official Name

CD40

Full Name

Cluster of differentiation 40

Alternative Names

CD40; Bp50; CDW40; TNFRSF5; p50; CD40 (protein); CD40 molecule

Gene ID

1000(Human); 21939(Mouse)

Uniprot ID

P25942 (Human); P27512 (Mouse)

References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Publications