Creative Biolabs

pLenti-CMV-MCS Lentiviral Expression Vector

[CAT#: NCC200570ZP]

Applications:
Virus Production
Cell Types:
Neural Lineage Cell

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Description
Lentiviral vectors based on human immunodeficiency virus 1 (HIV-1) have become the most commonly used vectors for gene transfer research. The dominant feature of lentiviral vectors is the ability of gene transfer and integration into dividing and non-divi. The pseudotype envelope with vesicular stomatitis virus envelope G (VSV-G) protein expands the range of target cells. Lentiviral vectors have been shown to deliver genes to neurons, lymphocytes, and macrophages, while previous retroviral vectors cannot use this cell type. Lentiviral vectors have also been shown to effectively transduce brain, liver, muscle and retina in vivo without toxicity or immune response. Recently, lentivirus systems have been widely used to effectively integrate siRNA into various cell lines and primary cells in vitro and in vivo.

Lentiviral particles are produced from 293T cells by transient transfection of plasmids encoding viral particle components. For safety considerations regarding HIV-1 infectivity, recent lentiviral packaging systems have divided the viral components into 3 or 4 plasmids.

The pLenti-CMV-MCS lentiviral expression vector contains multiple cloning sites (MCS) driven by the EF1α promoter. Contains multiple restriction sites to easily clone the promoter of your choice and the gene of interest.
Cell Types
Neural Lineage Cell
Species Reactivity
Human; Mouse; Rat
Applications
Virus Production
Research Areas
Stem Cell Research
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Room Temperature
Storage
Store at -20°C
Handling Advices
For all materials containing BSL-2 organisms, follow the recommended NIH guidelines. The NeuroShop Lentiviral Expression System is designed to minimize the chance of producing lentivirus with replication capabilities, but precautions should still be taken to avoid direct contact with viral supernatant.
Quality Control
This plasmid is sequence verified.
Shelf Life
This product is stable for 6 months when stored as directed.
Research Use Only
For research use only, not for diagnostic or therapeutic use.

For Research Use Only. Not For Clinical Use.
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