NeuroMab™ Mouse Anti-GFAP Monoclonal Antibody (CBP1571)

Product Overview

Description

Mouse anti-bovine GFAP antibody, clone 1B4 can recognize glial fibrillary acidic protein or GFAP, class III intermediate filaments. During the development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It involves various cell functions, such as cell structure and movement, cell communication and the function of the blood-brain barrier. It also plays a role in mitosis by regulating the network of filaments present in the cell. Deficiency of GFAP is the cause of Alexander disease, a rare central nervous system disease that mainly affects men. It is a progressive leukoencephalopathy that causes mental and physical development delays, dementia, seizures and early death.

Immunogen

Bovine spinal cord homogenate.

Species Reactivity

Bovine; Human; Mouse; Rat; Sheep; Dog; Porcine; Rabbit; Guinea Porcine; Chicken

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b

Clone Number

CBP1571

Applications

IHC-P; IHC-Fr; WB; RIA

Application Notes

IHC-P (1/200-1/1000)

Relevant Diseases

Alexander's Disease

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1360

Product Properties

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

GFAP

Official Name

GFAP

Full Name

Glial Fibrillary Acidic Protein

Alternative Names

Glial Fibrillary Acidic Protein; Intermediate Filament Protein; ALXDRD;

Gene ID

2670(Human); 14580(Mouse); 24387(Rat)

Uniprot ID

P14136(Human); P03995(Mouse); P47819(Rat)

References

1. Pegram, C.N. et al. (1985) Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species.
2. McLendon, R.E. et al. (1986) The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues.
3. Schwabenlander, M. et al. (2016) Brain, Craniofacial, and Dental Lesions of a Free-ranging Gray Wolf (Canis lupus) Implicated in a Human Attack in Minnesota, USA.

Publications