- Tracer Type:
- Anterograde tracing virus
- Applications:
- Neural Tracing
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Lot Number
SPECIFIC INQUIRY
inquiryDescription
Thymidine nucleotide kinase (TK) is an important part of HSV replication after nerve cell infection. In the presence of TK, HSV can replicate and express target genes in cells, and its progeny viruses will be transported to the synapse, and then through the synapse to enter downstream neurons, thereby achieving multi-synaptic tracking. After deleting TK (deleting TK), HSV-ΔTK can infect neurons but cannot replicate. We know that only viruses that can replicate have the ability to cross synapses, so HSV-ΔTK cannot cross synapses. However, if we provide AAV virus as an assistant for expressing TK protein, HSV-ΔTK can achieve anterograde single synaptic tracking.
Application
●Multi-synaptic tracking from the center of the brain to the periphery, or from lower to higher brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.
Tracer Type
Virus Type
Applications
Research Areas
Direction of Transport
Synaptic Restriction
Cell Types
Expression
Fluorophore
Storage
Research Use Only
References
2. Frazer M E, Hyghes J E, Mas trangelo M A, et al. Reduced pathology and improved behavioral performance in Alzheimer's disease mice vaccinated with HSV amplicons expressing amyloid-beta and interleukin-4[J]. Mol Ther, 2008, 16(5):845-853.
3. Coen, D.M., Kosz-Vnenchak, M., Jacobson, J.G., Leib, D.A., Bogard, C.L., Schaffer, P.A., Tyler, K.L., and Knipe, D.M. (1989). Thymidine kinase-negative herpes simplex virus mutants establish latency in mouse trigeminal ganglia but do not reactivate. Proceedings of the National Academy of Sciences of the United States of America 86, 4736-4740.
4. Li, X., Chen, W., Pan, K., Li, H., Pang, P., Guo, Y., Shu, S., Cai, Y., Pei, L., Liu, D., et al. (2018). Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus. Nature neuroscience 21, 1239-1250.
Publications (0)
ExcellentHighly recommended for detailed neural tracing studiesThe expression of the reporter gene was robust and consistent across different brain regions. The dual-reporter system allowed us to track both neural pathways and herpes simplex virus spread effectively. We were particularly impressed with the clarity of the fluorescence signal, which was bright and distinct, making our imaging analysis much easier.
ExcellentOur lab has been using various viral vectors for neural tracingThe tdtomato expression was very strong, and we observed minimal background noise. This product has significantly improved the quality of our neural mapping data, allowing us to identify previously unknown connections with great precision. The comprehensive user manual provided was also a great help.
ExcellentOverall, this product has exceeded my expectationsI used HSV-LSL-tdtomato-2a-TK(H356) for tracing long-range projections in the mouse brain. The tdtomato fluorescence was exceptionally bright, and the expression was specific to the targeted neurons. This made it easy to differentiate between traced and non-traced neurons. Additionally, the TK gene allowed for precise control over the viral spread, which was crucial for our experiments.
ExcellentThis product has greatly enhanced the accuracy and reproducibility of our experimentsThe tdtomato signal was consistently strong across multiple trials, and we encountered no issues with viral delivery. The combination of tdtomato and TK genes provides a versatile tool for both tracing and manipulating neural circuits.
ExcellentWe are very pleased with its performanceThe dual-reporter system allowed for detailed and accurate mapping of neural circuits. I also appreciated the technical support provided by the company, which was prompt and helpful. This product is a great investment for any neuroscience lab.
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