Product
Hot Products
Antibodies
- NeuroMab™ Anti-SEZ6 Antibody, Clone NR28P (Cat#: NRP-0422-P515)
- NeuroMab™ Rabbit Anti-Alpha-synuclein (CBP1631) (Cat#: NAB-08-PZ079)
- NeuroMab™ Anti-ApoC3 BBB Shuttle Antibody,Clone NR1738P (Cat#: NRZP-1022-ZP3503)
- NeuroMab™ Anti-SEZ6 Antibody, Clone NR30P (Cat#: NRP-0422-P517)
- NeuroMab™ Anti-Tau Antibody,Clone NR2946P (Cat#: NRP-0422-P1686)
- NeuroMab™ Anti-Tau Antibody,Clone NR2948P (Cat#: NRP-0422-P1683)
- NeuroMab™ Anti-Integrin αvβ8 BBB Shuttle Antibody,Clone NR2431P (Cat#: NRZP-1222-ZP1218)
- NeuroMab™ Anti-EPHB2 Antibody,Clone NR1370P (Cat#: NRP-0422-P1220)
- NeuroMab™ Anti-GARP Antibody,Clone NR3348P (Cat#: NRP-0422-P1639)
- NeuroMab™ Anti-pTau Antibody,Clone NR3595P (Cat#: NRP-0422-P1719)
Cells
- Rat Microglia Cell Line HAPI, Immortalized (Cat#: NCL2110P015)
- Rat Muller Cell (Cat#: NCL2110P040)
- Rat Immortalized Retinal Muller Cell Line rMC-1 (Cat#: NCL-2106-S93)
- Human Hippocampal Neuron Cells HPPNCs (Cat#: NCL2110P106)
- Human Blood Brain Barrier Model (Cat#: NCL-2103-P187)
- Human Glioblastoma Cell Line SF126 (Cat#: NCL-2108P35)
- Mouse Microglia Cell Line BV-2, Immortalized (Cat#: NCL2110P153)
- Human Microglia Cell Line HMC3, Immortalized (Cat#: NCL-2108P38)
- Human Brain Vascular Pericytes (Cat#: NCL-21P6-015)
- Human Retinal Epithelial Cell ARPE-19 (Cat#: NCL2110P069)
Kits
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
Tracers
- pAAV-hSyn-DIO-XCaMP-R-WPRE (Cat#: NTA-2012AD-P508)
- AAV2/9-hSyn-Flpo-EGFP-WPRE-pA (Cat#: NTA-2012-ZP149)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- AAV2/9-hEF1a-DIO-mCherry-P2A-TetTox-WPRE-pA (Cat#: NTA-2012-ZP268)
- pAAV-syn-FLEX-jGCaMP8f-WPRE (Cat#: NTA-2106-P064)
- Dextran, Cy5 Labeled, 2000 kDa (Cat#: NRZP-0722-ZP22)
- pAAV-syn-FLEX-jGCaMP8m-WPRE (Cat#: NTA-2106-P065)
- pAAV-syn-jGCaMP8s-WPRE (Cat#: NTA-2106-P063)
- AAV-EF1a-mCherry-flex-dtA (Cat#: NRZP-0622-ZP616)
- AAV2/9-hEF1a-fDIO-eNpHR 3.0-mCherry-WPRE-pA (Cat#: NTA-2012-ZP78)
Gene Related Products
- ABCA1 Antisense Oligonucleotide (AK311445) (Cat#: NV-2106-P27)
- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
Biospecimens
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
Cell Assay Reagents
- NeuroPro™ Anti-EPO BBB Shuttle Protein, HIRMab-EPO (Cat#: NRZP-0423-ZP508)
- NeuroPro™ Anti-ASA BBB Shuttle Protein, HIRMab-ASA (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-IDS BBB Shuttle Protein, HIRMab-IDS (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein, HIRMab-SGSH (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, HIRMab-GDNF (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, cTfRMAb-IDUA (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein, cTfRMAb-EPO (Cat#: NRZP-0423-ZP499)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, cTfRMAb-GDNF (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein, 8D3-IL-1RA (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, HIRMab-IDUA (Cat#: NRZP-0423-ZP502)
- Tracer Type:
- Anterograde tracing virus
- Applications:
- Neural Tracing
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
Herpes simplex virus (HSV) based on the H129 strain has been widely used as an anterograde tracer. The addition of fluorescent protein-modified HSV can not only effectively mark the connections between different brain regions, but also the connections between the surrounding and the center.
Thymidine nucleotide kinase (TK) is an important part of HSV replication after nerve cell infection. In the presence of TK, HSV can replicate and express target genes in cells, and its progeny viruses will be transported to the synapse, and then through the synapse to enter downstream neurons, thereby achieving multi-synaptic tracking. After deleting TK (deleting TK), HSV-ΔTK can infect neurons but cannot replicate. We know that only viruses that can replicate have the ability to cross synapses, so HSV-ΔTK cannot cross synapses. However, if we provide AAV virus as an assistant for expressing TK protein, HSV-ΔTK can achieve anterograde single synaptic tracking.
Application
●Multi-synaptic tracking from the center of the brain to the periphery, or from lower to higher brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.
Thymidine nucleotide kinase (TK) is an important part of HSV replication after nerve cell infection. In the presence of TK, HSV can replicate and express target genes in cells, and its progeny viruses will be transported to the synapse, and then through the synapse to enter downstream neurons, thereby achieving multi-synaptic tracking. After deleting TK (deleting TK), HSV-ΔTK can infect neurons but cannot replicate. We know that only viruses that can replicate have the ability to cross synapses, so HSV-ΔTK cannot cross synapses. However, if we provide AAV virus as an assistant for expressing TK protein, HSV-ΔTK can achieve anterograde single synaptic tracking.
Application
●Multi-synaptic tracking from the center of the brain to the periphery, or from lower to higher brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.
Tracer Type
Anterograde tracing virus
Virus Type
Herpes Simplex Virus (HSV)
Applications
Neural Tracing
Research Areas
Neural Circuit Mapping
Direction of Transport
Anterograde
Synaptic Restriction
Polysynaptic
Cell Types
Neuron
Expression
Normal Expression
Properites
Fluorophore
tdTomato
Storage
Please refer to the protocal.
Research Use Only
For research use only.
References
References
1. Bowers W J, Mastrangelo M A, Stanley H A, et al. HSV amplicon-mediated Abeta vaccination in Tg2576 mice:differential antigen-specific immune responses[J]. Neurobiol Aging, 2005,26(4):393-407.
2. Frazer M E, Hyghes J E, Mas trangelo M A, et al. Reduced pathology and improved behavioral performance in Alzheimer's disease mice vaccinated with HSV amplicons expressing amyloid-beta and interleukin-4[J]. Mol Ther, 2008, 16(5):845-853.
3. Coen, D.M., Kosz-Vnenchak, M., Jacobson, J.G., Leib, D.A., Bogard, C.L., Schaffer, P.A., Tyler, K.L., and Knipe, D.M. (1989). Thymidine kinase-negative herpes simplex virus mutants establish latency in mouse trigeminal ganglia but do not reactivate. Proceedings of the National Academy of Sciences of the United States of America 86, 4736-4740.
4. Li, X., Chen, W., Pan, K., Li, H., Pang, P., Guo, Y., Shu, S., Cai, Y., Pei, L., Liu, D., et al. (2018). Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus. Nature neuroscience 21, 1239-1250.
2. Frazer M E, Hyghes J E, Mas trangelo M A, et al. Reduced pathology and improved behavioral performance in Alzheimer's disease mice vaccinated with HSV amplicons expressing amyloid-beta and interleukin-4[J]. Mol Ther, 2008, 16(5):845-853.
3. Coen, D.M., Kosz-Vnenchak, M., Jacobson, J.G., Leib, D.A., Bogard, C.L., Schaffer, P.A., Tyler, K.L., and Knipe, D.M. (1989). Thymidine kinase-negative herpes simplex virus mutants establish latency in mouse trigeminal ganglia but do not reactivate. Proceedings of the National Academy of Sciences of the United States of America 86, 4736-4740.
4. Li, X., Chen, W., Pan, K., Li, H., Pang, P., Guo, Y., Shu, S., Cai, Y., Pei, L., Liu, D., et al. (2018). Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus. Nature neuroscience 21, 1239-1250.
Publications
Publications (0)
Scientific Resources
Customer Reviews and Q&As
Customer Reviews
Average Customer Ratings
Overall
5.0
ExcellentHighly recommended for detailed neural tracing studiesThe expression of the reporter gene was robust and consistent across different brain regions. The dual-reporter system allowed us to track both neural pathways and herpes simplex virus spread effectively. We were particularly impressed with the clarity of the fluorescence signal, which was bright and distinct, making our imaging analysis much easier.
ExcellentOur lab has been using various viral vectors for neural tracingThe tdtomato expression was very strong, and we observed minimal background noise. This product has significantly improved the quality of our neural mapping data, allowing us to identify previously unknown connections with great precision. The comprehensive user manual provided was also a great help.
ExcellentOverall, this product has exceeded my expectationsI used HSV-LSL-tdtomato-2a-TK(H356) for tracing long-range projections in the mouse brain. The tdtomato fluorescence was exceptionally bright, and the expression was specific to the targeted neurons. This made it easy to differentiate between traced and non-traced neurons. Additionally, the TK gene allowed for precise control over the viral spread, which was crucial for our experiments.
ExcellentThis product has greatly enhanced the accuracy and reproducibility of our experimentsThe tdtomato signal was consistently strong across multiple trials, and we encountered no issues with viral delivery. The combination of tdtomato and TK genes provides a versatile tool for both tracing and manipulating neural circuits.
ExcellentWe are very pleased with its performanceThe dual-reporter system allowed for detailed and accurate mapping of neural circuits. I also appreciated the technical support provided by the company, which was prompt and helpful. This product is a great investment for any neuroscience lab.
Q&As
How is the tdTomato fluorescence visualized, and what equipment is needed?
tdTomato fluorescence can be visualized using standard fluorescence microscopy equipment. The protein emits bright red fluorescence, making it easy to detect with a filter set optimized for red wavelengths (excitation: 554 nm, emission: 581 nm). Confocal or wide-field fluorescence microscopes with the appropriate filters will provide clear and detailed images of labeled neurons.
What are the expected transduction efficiency and expression levels in target neurons?
Transduction efficiency and expression levels can vary depending on factors such as target cell type, injection site, and viral titer. Generally, HSV vectors like HSV-LSL-tdTomato-2a-TK (H356) achieve high transduction efficiency in neurons, with robust expression of tdTomato visible within days post-injection. Optimize your experimental conditions to achieve the best results for your specific study.
For Research Use Only. Not For Clinical Use.
