HSV-ΔTK-LSL-tdTomato(H361)

[CAT#: NTA-2011-ZP19] Review(5) Q&As(3)

Cre dependent, Combined with AAV-TK, anterograde monosynaptic

Tracer Type:: Anterograde tracing virus

Applications:: Neural Tracing

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Product Overview

Description

Herpes simplex virus (HSV) based on the H129 strain has been widely used as an anterograde tracer. The addition of fluorescent protein-modified HSV can not only effectively mark the connections between different brain regions, but also the connections between the surrounding and the center.

Thymidine nucleotide kinase (TK) is an important part of HSV replication after nerve cell infection. In the presence of TK, HSV can replicate and express target genes in cells, and its progeny viruses will be transported to the synapse, and then through the synapse to enter downstream neurons, thereby achieving multi-synaptic tracking. After deleting TK (deleting TK), HSV-ΔTK can infect neurons but cannot replicate. We know that only viruses that can replicate have the ability to cross synapses, so HSV-ΔTK cannot cross synapses. However, if we provide AAV virus as an assistant for expressing TK protein, HSV-ΔTK can achieve anterograde single synaptic tracking.

Application
●Multi-synaptic tracking from the center of the brain to the periphery, or from lower to higher brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.

Tracer Type

Anterograde tracing virus

Virus Type

Herpes Simplex Virus (HSV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Direction of Transport

Anterograde

Synaptic Restriction

Polysynaptic

Cell Types

Neuron

Expression

Normal Expression

Properites

Fluorophore

tdTomato

Storage

Please refer to the protocal.

Research Use Only

For research use only.

References

1. Bowers W J, Mastrangelo M A, Stanley H A, et al. HSV amplicon-mediated Abeta vaccination in Tg2576 mice:differential antigen-specific immune responses[J]. Neurobiol Aging, 2005,26(4):393-407.
2. Frazer M E, Hyghes J E, Mas trangelo M A, et al. Reduced pathology and improved behavioral performance in Alzheimer's disease mice vaccinated with HSV amplicons expressing amyloid-beta and interleukin-4[J]. Mol Ther, 2008, 16(5):845-853.
3. Coen, D.M., Kosz-Vnenchak, M., Jacobson, J.G., Leib, D.A., Bogard, C.L., Schaffer, P.A., Tyler, K.L., and Knipe, D.M. (1989). Thymidine kinase-negative herpes simplex virus mutants establish latency in mouse trigeminal ganglia but do not reactivate. Proceedings of the National Academy of Sciences of the United States of America 86, 4736-4740.
4. Li, X., Chen, W., Pan, K., Li, H., Pang, P., Guo, Y., Shu, S., Cai, Y., Pei, L., Liu, D., et al. (2018). Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus. Nature neuroscience 21, 1239-1250.

Publications

Publications (0)

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Scientific Resources

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Virus Vector Tools for Neural Cell Manipulation

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The vector was instrumental in our recent neural tracing studies

The tdTomato fluorescence was robust and easily detectable, even in densely packed neural tissues. The conditional LSL system worked flawlessly, ensuring that the reporter was only activated in the desired cell populations. This specificity significantly reduced background noise and made our results much more reliable.

Excellent

This tool has become a staple in our lab for neural circuit mapping

We have been using HSV-ΔTK-LSL-tdTomato for tracing neuronal pathways in mouse models, and the results have been consistently excellent. The virus's efficiency in infecting neurons and the strong expression of tdTomato allowed us to visualize intricate neural networks clearly.

Excellent

An excellent product for detailed neural studies

We integrated HSV-ΔTK-LSL-tdTomato into our research on synaptic connections, and the results were outstanding. The viral vector demonstrated high transduction efficiency, and the tdTomato fluorescence provided clear and detailed images of neuronal pathways. This tool has greatly facilitated our understanding of neural networks.

Excellent

This vector is a reliable and powerful tool for neural research

Our research on neural pathways has greatly benefited from using HSV-ΔTK-LSL-tdTomato. The vector demonstrated high efficiency in infecting neurons. The conditional system worked as expected, providing precise control over gene expression. It's a highly effective tool for neural tracing studies.

Excellent

Unprecedented clarity in our neural tracing experiments

The tdTomato signal was exceptionally bright, making it easy to track neuronal pathways. The conditional expression system was highly effective, ensuring precise targeting of specific neurons.

Q&As

What are the benefits of using tdTomato as the reporter in this vector?

Using tdTomato as the reporter in this vector offers several advantages. TdTomato provides bright and stable fluorescence, which is critical for long-term imaging and tracking. It also has a higher photostability and quantum yield compared to other fluorescent proteins, allowing for more precise visualization of neuronal structures. Additionally, its red fluorescence reduces overlap with commonly used green markers, facilitating multicolor experiments.

What precautions should be taken when handling the HSV-ΔTK-LSL-tdTomato (H361) vector in the laboratory?

When handling the HSV-ΔTK-LSL-tdTomato (H361) vector, it is essential to follow biosafety guidelines appropriate for BSL-2 agents. This includes using personal protective equipment, such as gloves and lab coats, and working in a biosafety cabinet to prevent contamination. Proper waste disposal and decontamination procedures should also be followed to ensure safety and compliance with laboratory standards.

What are the optimal conditions for injecting the HSV-ΔTK-LSL-tdTomato (H361) vector into the brain, and what factors should be considered?

The optimal conditions for injecting the HSV-ΔTK-LSL-tdTomato (H361) vector involve precise stereotaxic surgery to accurately target specific brain regions. Factors to consider include the volume and concentration of the viral solution, the speed of injection, and the choice of anesthesia to minimize tissue damage. Proper post-operative care and monitoring of the subjects are also critical to ensure successful expression and recovery.