HSV-ΔTK-hUbC-tdTomato

Description

Herpes simplex virus (HSV) based on the H129 strain has been widely used as an anterograde tracer. The addition of fluorescent protein-modified HSV can not only effectively mark the connections between different brain regions, but also the connections between the surrounding and the center.

Thymidine nucleotide kinase (TK) is an important part of HSV replication after nerve cell infection. In the presence of TK, HSV can replicate and express target genes in cells, and its progeny viruses will be transported to the synapse, and then through the synapse to enter downstream neurons, thereby achieving multi-synaptic tracking. After deleting TK (deleting TK), HSV-ΔTK can infect neurons but cannot replicate. We know that only viruses that can replicate have the ability to cross synapses, so HSV-ΔTK cannot cross synapses. However, if we provide AAV virus as an assistant for expressing TK protein, HSV-ΔTK can achieve anterograde single synaptic tracking.

Application
●Multi-synaptic tracking from the center of the brain to the periphery, or from lower to higher brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.

Tracer Type

Anterograde tracing virus

Virus Type

Herpes Simplex Virus (HSV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Direction of Transport

Anterograde

Promoter

UBC

Expression

Specific Expression

Fluorophore

dTomato

Storage

Please refer to the protocal.

Research Use Only

For research use only.

References

1. Bowers W J, Mastrangelo M A, Stanley H A, et al. HSV amplicon-mediated Abeta vaccination in Tg2576 mice:differential antigen-specific immune responses[J]. Neurobiol Aging, 2005,26(4):393-407.
2. Frazer M E, Hyghes J E, Mas trangelo M A, et al. Reduced pathology and improved behavioral performance in Alzheimer's disease mice vaccinated with HSV amplicons expressing amyloid-beta and interleukin-4[J]. Mol Ther, 2008, 16(5):845-853.
3. Coen, D.M., Kosz-Vnenchak, M., Jacobson, J.G., Leib, D.A., Bogard, C.L., Schaffer, P.A., Tyler, K.L., and Knipe, D.M. (1989). Thymidine kinase-negative herpes simplex virus mutants establish latency in mouse trigeminal ganglia but do not reactivate. Proceedings of the National Academy of Sciences of the United States of America 86, 4736-4740.
4. Li, X., Chen, W., Pan, K., Li, H., Pang, P., Guo, Y., Shu, S., Cai, Y., Pei, L., Liu, D., et al. (2018). Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus. Nature neuroscience 21, 1239-1250.