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Creative Biolabs

Human LRP5 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP146]

Human LRP5 knockout cell lysate

Species:
Human
Cell Types:
Other Cells

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human LRP5 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 5 bp deletion in exon 2.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

LRP5

Official Name

LRP5

Full Name

Low-density lipoprotein receptor-related protein 5

Alternative Names

LRP5; BMND1; EVR1; EVR4; HBM; LR3; LRP-5; LRP7; OPPG; OPS; OPTA1; VBCH2; LDL receptor related protein 5; PCLD4; LRP-7

Gene ID

4041(Human); 16973(Mouse); 293649(Rat)

Uniprot ID

O75197(Human); Q91VN0(Mouse)
Publications

Publications (0)

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