Human MANF Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP221]

Human MANF knockout cell lysate

Species:: Human

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This is a HEK293T cell transfected lysate in which Human MANF has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and 25 bp deletion in exon 1 and 4 bp insertion in exon 1.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmitter

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

MANF

Official Name

MANF

Full Name

mesencephalic astrocyte-derived neurotrophic factor

Alternative Names

MANF; ARMET; ARP; arginine-rich; mutated in early-stage tumors; arginine-rich protein; mesencephalic astrocyte-derived neurotrophic factor; mesencephalic astrocyte derived neurotrophic factor