NeuroMab™ Anti-Amyloid Beta 16-28 BBB Shuttle Antibody(NRZP-1022-ZP3660)

Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

In Vitro; ELISA

Relevant Diseases

Alzheimer's Disease

Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only

Target

AmyloidBeta16-28

Official Name

Amyloid Beta

Full Name

Amyloid Beta

Alternative Names

Aβ; Abeta

Product Pictures

ELISA

Figure 1 shows the epitope mapping of antibody 9TL by peptide competition.

A(31-ao peptides were immobilized on SA chips. Monoclonal antibody 2289 and 9TL Fab fragments (50 nM each), each mixed with 10 gM of each peptide (amino acids 28-40, 1-40, 1-28, 28- 42, 22-35, 1-16, 1-43, 33-40, 1-38, or 17-40 AP) or no peptide and flowed onto the chip. Antibody Fab fragments immobilized API-40 peptide were measured.

Figure 2 is a graph showing epitope mapping of antibody 2H6 by peptide competition. A(31_40 peptide immobilized on SA chip. Monoclonal antibodies 2289, 2286 or 2H6 (100 inM each), each pre-incubated with 16 gM for 1 hr

Various peptides (amino acids 1-16, 1-28, 1-38, 1-40, 1-42, 1-43, 17-40, 17-42, 22-35, 25-35 or 33-40 A( 3) Or no peptide flows to the chip.Measure the binding of antibody to immobilized A (31_40 peptide).

ELISA

Figure 3 is a graph showing the binding of antibodies 2H6, 2286 and 2289 to different A (3 peptide C-terminal variants). GST-A (3 variants (M35A, V36A, G37A, G38A, V39A, or V40A), or GST-A (3 peptides 1-39, 1-41, 1-40, 1-42) were immobilized on ELISA plates.

Monoclonal antibodies 2286, 2H6 or 2289 (0.3 nM per mAb) were incubated with each immobilized peptide and their binding was detected by further incubation with biotinylated anti-mouse IgG.

Publications