NeuroMab™ Rat Anti-PPP2R1A Monoclonal Antibody (CBP4377)

Description

Protein phosphatase 2A (PP2A) is a heterotrimeric enzyme and is the most abundant phosphatase specific for serine- and threonine phosphorylation in mammalian cells. PP2A consists of a dimeric core enzyme composed of the structural A and catalytic C subunits, and a regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. The A subunit, a founding member of the HEAT repeat protein family (huntington-elongation-A subunit-TOR), is the scaffold required for the formation of the heterotrimeric complex. When the A subunit binds it alters the enzymatic activity of the catalytic subunit, even if the B subunit is absent. Multicellular eukaryotes express four classes of variable regulatory subunits: B (PR55), B' (B56 or PR61), B″ (PR72), and B-´ (PR93/PR110), with at least 16 members in these subfamilies. In addition, accessory proteins and posttranslational modifications (such as methylation) control PP2A subunit associations and activities. Dephosphorylation of substrates targeted by PP2A plays a vital role in a large variety of cellular processes including signal transduction, regulation of the cell cycle, gene expression and development.

Immunogen

This monoclonal antibody was raised against human Aα subunit 7.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a

Clone Number

CBP4377

Applications

WB; IP

Application Notes

WB (1.0 - 5.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

1 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

PPP2R1A

Official Name

PPP2R1A

Full Name

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Alternative Names

PPP2R1A; PP2A-Aalpha; PP2AAALPHA; PR65A; MRD36; protein phosphatase 2 scaffold subunit Aalpha; PP2AA

Gene ID

Uniprot ID