pAAV-GfaABC1D-NES-jRGECO1a-WPRE

Product Overview

Tracer Type

Calcium Sensor

Functional Gene

jRGECO1a

Virus Type

Adeno-Associated Virus (AAV)

Applications

Calcium Sensor

Sub Type

AAV packing

Research Areas

Neural Circuit Mapping

Promoter

GfaABC1D

Expression

Normal Expression

Properites

Shipping

Viral particles are shipped frozen on dry ice.

Handling Advice

The lentivirus is generally referred to as BSL-2. AAV is usually considered BSL-1, but may require BSL-259 handling depending on the insert.

Storage

Store at -80°C. Thaw just before use and keep on ice.

Quality Control

Creative Biolabs ensures high-quality viral vectors by optimizing and standardizing production plans and implementing strict quality control (QC). The specific QC analysis performed for each virus batch is different.

Titer: The precise titer of the sample will be reported on the test tube. The potency you see on this page is the guaranteed minimum potency.

Research Use Only

For research use only.

Publications

Publications (0)

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Scientific Resources

[Flyer]

Ready-to-Use AAV Biosensors

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[Knowledge Sharing]

Applications of Calcium Imaging Technology in Nervous System Research

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The specificity and efficiency of the sensor are top-notch

The sensor provides precise and consistent readings, and its integration with our AAV delivery system was seamless.

Excellent

It has greatly facilitated our process

We've been using the pAAV-GfaABC1D-NES-jRGECO1a-WPRE in our calcium imaging experiments for several months. The sensor’s high sensitivity to calcium fluctuations has allowed us to observe subtle changes in intracellular calcium levels with remarkable clarity.

Excellent

We’ve achieved unprecedented clarity

This calcium sensor is exceptional for our experiments on neuronal calcium dynamics. The signal-to-noise ratio is impressive, and the jRGECO1a variant offers great stability and brightness.

Excellent

Robust signal detection capabilities

Incorporating the pAAV-GfaABC1D-NES-jRGECO1a-WPRE sensor into our experiments has been a breeze. The sensor’s performance in detecting calcium transients is outstanding.

Excellent

A huge success

Its high-resolution calcium measurements have provided us with critical data on cellular responses under various conditions.

Q&As

How does the sensor perform in different cell types? Are there any specific considerations?

The pAAV-GfaABC1D-NES-jRGECO1a-WPRE sensor is versatile and performs well in a range of cell types, including neurons, muscle cells, and various types of cancer cells. However, optimal performance may depend on factors such as cell health and expression levels. It’s important to validate the sensor’s expression in your specific cell type and ensure that the AAV delivery system is compatible with your cells to achieve the best results.

Can the sensor be used in both fixed and live-cell imaging experiments?

The sensor is primarily designed for live-cell imaging, where it can monitor real-time calcium dynamics. While it is optimized for live-cell applications, it can also be used in fixed-cell imaging to study calcium signaling patterns post-fixation. However, the sensor's primary strength lies in its ability to provide dynamic, real-time data during live-cell experiments.

What are some common troubleshooting steps if the sensor’s signal appears weak or inconsistent?

If you encounter weak or inconsistent signals, first check the expression levels of the sensor and ensure that it is properly integrated into your cells. Verify that your microscopy setup is correctly calibrated and that the excitation and emission settings are optimized for the sensor. Additionally, ensure that the cells are healthy and that no experimental conditions are affecting the calcium dynamics or sensor performance.