SSEA-1 NL493-Conjugated Live Cell Antibody Kit (Human&Mouse)

[CAT#: NCC200519ZP]

For the detection of SSEA-1 expression in live cells by immunocytochemical staining.

Applications:: Live Cell Imaging

Cell Types:: iPSC

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Product Overview

Description

Maintaining pluripotent human embryonic stem cells and inducing pluripotent stem cells requires colony selection and cell expansion. It is often time-consuming and laborious to select colonies containing pluripotent stem cells by manually analyzing the colony morphology. In addition, morphology-based colony selection cannot consistently guarantee a pluripotent starting population because cells may not be completely reprogrammed or may naturally differentiate within the colony.

To address this issue, we provided GloLIVE ™ antibodies, which enable researchers to confirm the expression of stem cell markers in live cells before selecting colonies.

Main benefits
Verify marker expression in live stem cells
Just keep warm for 30 minutes
Allow cell culture to continue after staining
Cell proliferation or stemness is not affected

Cell Types

iPSC

Species Reactivity

Human; Mouse

Applications

Live Cell Imaging

Application Notes

Real-time pluripotent stem cell imaging:
Promote the selection of high-quality, undifferentiated stem cell colonies to reduce experimental differences.
Verify the pluripotency of stem cells within 30 minutes to ensure effective use of time and reagents.
Allow the continuous cultivation of pluripotent cells without adversely affecting the proliferation or dryness of the stained cells.
If a 2 mL staining volume is used, the antibody can be used for 12 tests.

Relevant Diseases

Neurological Disorders

Research Areas

Stem Cell Research

Properties

Components

NL493-conjugated mouse anti-human / mouse SSEA-1 monoclonal antibody
Supplied with 50-fold antibody in 0.5 mL PBS

Formulation

Azide free

Endotoxin Level

5 EU / mL

Form

Liquid

Storage

Store unopened products at 2-8 ° C. Avoid light.

Shelf Life

Stable until expiry date (EXP) on label.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

SSEA-1

Official Name

FUT4

Full Name

Stage-specific Embryonic Antigen-1

Alternative Names

alpha-(1;3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1;3) fucosyltransferase; myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1; CD15; FCT3A; ELFT; SSEA-1; LeX

Publications