Human FGFR4 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP341]

Human FGFR4 knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human FGFR4 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neural Cell

Marker

Neural Stem Cell

Species

Human

Properties

Cell State

Cell lysate

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

FGFR4

Official Name

FGFR4

Full Name

Fibroblast Growth Factor Receptor 4

Alternative Names

Fibroblast Growth Factor Receptor 4; EC 2.7.10.1; JTK2; TKF; Tyrosine Kinase Related To Fibroblast Growth Factor Receptor; Hydroxyaryl-Protein Kinase; Protein-Tyrosine Kinase;