Human KCNQ1 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP178]

Human KCNQ1 knockout cell lysate

Species:: Human

Cell Types:: HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human KCNQ1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon6 and 64 bp deletion in exon6.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Pathways

Potassium Signaling

Species

Human

Properties

Form

There are 2 isoforms produced by alternative splicing. Isoform 2 also known as: TKvLQT1.

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

KCNQ1

Official Name

KCNQ1

Full Name

Potassium Voltage-Gated Channel Subfamily Q Member 1

Alternative Names

Potassium Voltage-Gated Channel Subfamily Q Member 1; IKs Producing Slow Voltage-Gated Potassium Channel Subunit Alpha KvLQT1; Potassium Channel; Voltage Gated KQT-Like Subfamily Q; Member 1; Potassium Voltage-Gated Channel; KQT-Like Subfamily; Member 1; Voltage-Gated Potassium Channel Subunit Kv7.1; KVLQT1; KCNA8; KCNA9; Potassium Voltage-Gated Channel Subfamily KQT Member 1; Kidney And Cardiac Voltage Dependend K+ Channel; Slow Delayed Rectifier Channel Subunit; Jervell And Lange-Nielsen Syndrome 1;