Human PPP2R5E Knockout HeLa Cell Lysate
[CAT#: NCL2008ZP390]
Human PPP2R5E knockout cell lysate
- Species:
- Human
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This is a HeLa cell transfected lysate in which Human PPP2R5E has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
Other Cells
Cell Location
Epithelial
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Research Areas
Neurotransmission
Pathways
Intracellular Signaling
Species
Human
Properties
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target Details
Target
PPP2R5E
Official Name
PPP2R5E
Full Name
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform
Alternative Names
PPP2R5E; protein phosphatase 2 regulatory subunit B'epsilon; B56epsilon; B56E
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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