NeuroMab™ Anti-BKBeta2 potassium channel subunit BBB Shuttle Antibody, Clone N53/32

Product Overview

Description

The BBB is a highly selective membrane that surrounds the blood vessels in the brain, and it can prevent many molecules, including potentially life-saving drugs, from entering the brain. The anti-KCNMB2 BBB Shuttle Antibody is designed to bind to receptors on the surface of cells in the BBB, and once bound, the antibody can cross the BBB and into the brain. This technology has the potential to revolutionize the treatment of brain disorders by enabling drugs to reach the brain more effectively.

Immunogen

Fusion protein amino acids 1-41 (MFIWTSGRTSSSYRQDEKRNIYQKIRDHDLLDKRKTVTALK, cytoplasmic N-terminus) and 218-235 (KLTQYLSLLCERIQRINR, cytoplasmic C-terminus) of mouse BKBeta2 (also known as BK channel subunit beta-2, Calcium-activated potassium channel subunit beta-2, Calcium-activated potassium channel, subfamily M subunit beta-2, Charybdotoxin receptor subunit beta-2, K(VCA)beta-2, Maxi K channel subunit beta-2, Slobeta-2 and Kcnmb2, accession number Q9CZM9)
Human: 97% and 100% identity (40/41 and 18/18 amino acids identical, respectively)
Rat: 97% and 100% identity (40/41 and 18/18 amino acids identical, respectively)
<50% identity with other BKBeta subunits

Construction

We offer several options for BBB shuttle antibody design. Antibody construction can be customized according to customer requirements.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

N53/32

Applications

WB; IHC; ICC

Application Notes

No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4

Research Areas

K+ channels

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

KCNMB2

Official Name

KCNMB2

Full Name

BKbeta2 K+ channel

Alternative Names

KCNMB2; potassium calcium-activated channel subfamily M regulatory beta subunit 2

Uniprot ID

Q9CZM9

Product Pictures

Fig. 1 Immunoblot against extracts of COS cells transiently transfected with GFP-tagged BKBeta2 or untagged Kv2.1 plasmid and probed with N53/32 (left) or K89/41 (right) TC supe.

Fig. 2 Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N53/32 (left) or N52A/42 (right) TC supe.

Publications