Product
Human EPHB4 Knockout HEK293T Cell Lysate
[CAT#: NCL2008ZP82]
Human EPHB4 knockout cell lysate
- Species:
- Human
- Cell Types:
- HEK293T
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This is a HEK293T cell transfected lysate in which Human EPHB4 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HEK293T
Cell Location
Epithelial
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Research Areas
Neurotransmission
Pathways
Intracellular Signaling
Marker
Tyrosine Kinase Receptors
Species
Human
Properties
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12
Tissue Source
Kidney
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target Details
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
