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Creative Biolabs

Human GCH1 Knockout A549 Cell Lysate

[CAT#: NCL2008ZP210]

Human GCH1 knockout cell lysate

Species:
Human
Cell Types:
Other Cells

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Product Overview

Description

This is a A549 cell transfected lysate in which Human GCH1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp deletion in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Pathways

Nitric Oxide Signaling

Marker

Nitric Oxide

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X,Y
D5S818: 11
D13S317: 11
D7S820: 8, 11
D16S539: 11, 12
vWA: 14
TH01: 8,9.3
TPOX: 8,11
CSF1PO: 10, 12

Tissue Source

Lung

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

GCH1

Official Name

GCH1

Full Name

GTP Cyclohydrolase 1

Alternative Names

GTP Cyclohydrolase 1; GTP Cyclohydrolase I; Dystonia 14; EC 3.5.4.16; GTP-CH-I; DYT5; GCH; Guanosine 5-Triphosphate Cyclohydrolase I;

Gene ID

2643(Human); 14528(Mouse); 29244(Rat)

Uniprot ID

P30793(Human); Q05915(Mouse); P22288(Rat)
Publications

Publications (0)

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