Human GCH1 Knockout A549 Cell Lysate
[CAT#: NCL2008ZP210]
Human GCH1 knockout cell lysate
- Species:
- Human
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This is a A549 cell transfected lysate in which Human GCH1 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
Other Cells
Cell Location
Epithelial
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp deletion in exon1.
Passage Number
<20
Knockout Validation
Sanger Sequencing
Research Areas
Neurotransmission
Pathways
Nitric Oxide Signaling
Marker
Nitric Oxide
Species
Human
Properties
Cell State
Cell lysate
STR Analysis
Amelogenin X,Y
D5S818: 11
D13S317: 11
D7S820: 8, 11
D16S539: 11, 12
vWA: 14
TH01: 8,9.3
TPOX: 8,11
CSF1PO: 10, 12
D5S818: 11
D13S317: 11
D7S820: 8, 11
D16S539: 11, 12
vWA: 14
TH01: 8,9.3
TPOX: 8,11
CSF1PO: 10, 12
Tissue Source
Lung
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target Details
Target
GCH1
Official Name
GCH1
Full Name
GTP Cyclohydrolase 1
Alternative Names
GTP Cyclohydrolase 1; GTP Cyclohydrolase I; Dystonia 14; EC 3.5.4.16; GTP-CH-I; DYT5; GCH; Guanosine 5-Triphosphate Cyclohydrolase I;
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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