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Creative Biolabs

Human L1CAM Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP277]

Human L1CAM knockout cell lysate

Species:
Human
Cell Types:
Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human L1CAM has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 154 bp insertion in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Neurogenesis

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

CD171

Official Name

L1CAM

Full Name

L1 cell adhesion molecule

Alternative Names

L1CAM; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1; L1 cell adhesion molecule

Gene ID

3897(Human)

Uniprot ID

P32004(Human)
Publications

Publications (0)

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