Transgenic Cell Line Models

Creative Biolabs is a leading service provider that focuses on neuroscience research. Now we have established a novel platform to generate various transgenic cell line models to serve our customers. We are confident in offering the best and most suitable service for our customers all over the world.

Background of Transgenic Cell Line

Transfection is a gene transfer technology to introduce nucleic acids into eukaryotic cells. In the past, it is a basic method to study protein expression and gene functions in a cellular environment. Now it has been used for the generation of transgenic cell line models. For example, DEAE-dextran is a cationic polymer used to transfect cultured mammalian cells, while calcium phosphate co-precipitation is the method for both transient and stable transfection in multiple cell types. Compared with transient transfection, the stable transgenic cell lines present excellent cell homogeneity.

Fig.1 Vector maps used to generate model cell lines. (Grishchenko, 2021)

Human pluripotent stem cells (hPSCs), especially the induced pluripotent stem cells (iPSCs) from patients, provide a novel research tool to understand disease pathogenesis. Genetic modification and gene targeting are key steps to further obtain the potential of hPSCs. However, the transgenic efficiency of hPSCs is low and the transgene expression is unstable. Compared with transgenic animals, it is always difficult to generate transgenic hPSCs. Based on years of experience in neuroscience research, Creative Biolabs can establish transgenic hPSC and human embryonic stem cell (hESC) lines with reporters using CRISPR/CAS-mediated homologous recombination technology. We can also screen key molecules through transgenic cell lines to facilitate the development of therapeutic strategies against neurodegenerative diseases.

Protocol for Transgenic Cell Line Generation

The interested cDNA can be amplified from total RNAs extracted from human tissues with RT-PCR. And then the obtained cDNA would be cloned into the pGEM-T vector. The cDNA fragments can be identified by DNA sequencing and subcloned into mammalian expression vectors. Finally, transfection of Chinese hamster lung (CHL) cells with recombinant plasmids allowed the establishment of transgenic cell lines.

Features of Our Service

• Advanced platforms to ensure the best achievement for every single step
• Extensive experience
• Multiple pathways to enhance the success rate
• Reliable lab report with timely updates
• Affordable price with high quality

As a global company with extensive scientific expertise, we guide clients from discovery to approval and provide continuity for their entire program. If you are interested in our neurological products or services, please do not hesitate to contact us for more details. We are capable of meeting the specific need of each customer with the best project design.

Reference

1. Grishchenko, I.V.; et al. A transgenic cell line with inducible transcription for studying (CGG) n repeat expansion mechanisms. Vavilov Journal of Genetics and Breeding. 2021, 25(1): 117.